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TECHSPEC® components are designed, specified, or manufactured by Edmund Optics. TECHSPEC® components are designed, specified, or manufactured by Edmund Optics. Learn More

Fluorescence Filter Sets

Fluorescence Filter Sets

Fluorescence Filter Sets Fluorescence Filter Sets
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  • Excitation, Emission, and Dichroic Filters for Fluorescence Imaging
  • >93% Transmission and OD 6 Blocking for Maximum Brightness and Contrast
  • Hard Sputtered Coatings on Single Substrate
  • TECHSPEC Pre-Mounted Filter Cubes Also Available

Our TECHSPEC® Fluorescence Filter Sets are designed to provide brilliant images when integrated into a fluorescence microscope. The high transmission of the bandpass filters guarantees the brightest possible images, while the deep blocking ensures no unwanted light gets to the detector. This ensures images will be brighter and will have much greater signal-to-noise than what is commonly available with soft coated, evaporated filters. TECHSPEC Fluorescence Filter Sets are ideal for fluorescence microscopy or fluorescence imaging applications and are available for common fluorescent dyes and fluorescent proteins. Additionally these filters are also used in polymerase chain reaction (PCR) diagnostic instruments.

Common Specifications

Substrate:
Fused Silica (Corning 7980)
Coating:
Hard Coated

Products

 Compatible Fluorophore   Excitation Wavelength (nm)   Emission Wavelength (nm)   Compare   Stock Number   Price  Buy
CFP 426 - 450 467 - 498
GFP 457 - 487 502 - 538
FITC 467 - 498 513 - 556
YPF 488 - 512 532 - 554
CY3 513 - 556 570 - 613
TRITC 532 - 554 570 - 613
Texas Red 542 - 582 604 - 644
mCherry 542 - 582 603 - 678
CY5 604 - 644 672 - 712
CY5.5 635 - 675 696 - 736
DAPI (High Brightness) 352 - 402 417 - 477
DAPI (High Contrast) 382 - 393 417 - 477
Lucifer Yellow 426 - 450 513 - 556
SpectrumGreen 484 - 504 517 - 537
Qdot® 525 415 - 455 518 - 533
Qdot® 605 415 - 455 598 - 613
Qdot® 655 415 - 455 648 - 663
SpectrumGold 524 - 544 558 - 586
SpectrumRed 576 - 596 612 - 644
Indocyanine Green (ICG) 748 - 789 814 - 851

Product Details

Fluorescence filter sets include an Excitation and Emission filter from our TECHSPEC® Fluorescence Bandpass Filters and a Dichroic filter from our TECHSPEC® Dichroic Filters. Excitation filters are placed within the illumination path of fluorescence microscopes to pass only wavelengths within the excitation range of the fluorophore under inspection. Emission filters are placed within the imaging path of a fluorescence microscope to pass only wavelengths within the emission range of the fluorophore. Dichroic filters are placed in between the excitation filter and the emission filter to reflect the excitation signal towards the fluorophore while transmitting the emission signal to the detector.

Filter sets are listed with the most common fluorophores. For a full list of compatible fluorophores with each set, view Fluorophores and Optical Filters for Fluorescence Microscopy. TECHSPEC Fluorescence Filter Sets are available pre-mounted in Olympus or Nikon Filter Cubes.

Technical Information

 
  Excitation and Emission Filters Dichroic Filter
Size 25.0mm 25.2 x 35.6mm
Thickness 3.5mm 1.05mm
Tolerances
Size +0.0/-0.1
Thickness ±0.1mm
Clear Aperture 22.0mm 22.0 x 30.0mm
Transmitted Wavefront (RMS) <λ/4
Angle of Incidence 0.0° ±5.0° 45.0° ±5.0°
Compatible Microscopes Most common Nikon, Olympus, and Zeiss Microscopes