TECHSPEC® 120i Plan APO Infinity Corrected Objectives are infinite conjugate microscope objectives designed to reduce the overall weight and size of an imaging system while maintaining optical performance. These objectives allow for a reduction in overall system length, by eliminating the requirement for the objectives to be parfocal to each other. TECHSPEC® 120i Plan APO Infinity Corrected Objectives feature c-mount threading to provide easy integration into a wide range of machine vision and life science systems. These objectives are ideal for biomedical and machine vision applications such as microscopy, flow cytometry, pharmaceutical inspection, and assembly line or fault inspection.
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The minimum required components for a basic digital microscope system are a TECHSPEC® 120i Plan APO Infinity Corrected Objective, camera tube, tube lens, lens tube spacer, and an industrial C-Mount camera (Figure 1). Learn more about assembling a basic system in either a vertical or horizontal configuration here.
For life science systems requiring fluorescence imaging, TECHSPEC® Fluorescence Filter Sets can also be incorporated into the system with the available accessories. Learn more about assembling these fluorescence configurations here.
Numerical aperture (NA) is an important specification to define for a microscope objective because it describes the objective’s ability to gather light at a fixed working distance. The NA is often assumed to describe an objective’s resolving power; however, this is not an accurate assumption. The equation below is the first-order equation used to calculate NA. This equation is useful for understanding NA on-axis, but it does not consider field changes for off-axis performance. In addition, this equation does not consider tolerances during the manufacturing and assembly of microscope objectives that can impact the performance.
n: refractive index of imaging medium
θ: one-half of the objective’s opening angle
Figure 1 below demonstrates the off-axis performance of the 5X 120i Plan APO Infinity Corrected Objective compared to another 5X Plan APO infinity corrected objective from another manufacturer that has the same NA of 0.14. Performance off-axis is better with the 120i Objective in the corner of a 1.1” sensor, indicating that NA is not the defining factor for the performance of an objective.
Figure 4. Contrast Comparison using a USAF Resolution Test Target on a 1.1” Sensor
a) Image with 5X 120i Plan APO Infinity Corrected Objective showing 20% contrast at 330 lp/mm
b) 5X Plan APO Infinity Corrected Objective from another manufacturer showing 10% contrast at 330 lp/mm
Learn how to assemble a microscope system using the award-winning Edmund Optics® 120i Plan APO Infinity Corrected Objectives
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